CHO-K1 Human CCR5 Cell Line
Cat. No： RQP71352
Size： 1 vial of frozen cells (>1E6 per vial in 1 mL)
Unit Price： Contact For Pricing
Description
Product Info
Biological Info
Assay Data
Cell Culture
This product is a CHO-K1 stable cell line that stably expresses tag-free human CCR5 receptor. Upon agonist binding to CCR5, the Gi-type G protein signaling pathway is activated, inhibiting adenylyl cyclase and triggering a decrease in intracellular cAMP concentration. Therefore, receptor activity can be easily quantified in live cells in a dose-response manner by measuring changes in cAMP levels. This cell line is suitable for high-throughput screening (HTS) and receptor functional evaluation assays.
Figure 1. Schematic diagram of CCR5 CHO-K1 cell model
Assay Principle
Cells are stimulated with test compounds to modulate intracellular cAMP levels. The resulting cAMP changes are then detected through a competitive immunoassay format, and receptor activity is quantified by dose-response analysis.
| Cat. No | RQP71352 |
| Product Name | CHO-K1 Human CCR5 Cell Line |
| Engineering method | plasmid transfection |
| Culture Properties | Adherent |
| Stability | 32passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Mycoplasma Status | Negative |
| Culture Medium | F12K+10%FBS+5μg/ml puromycin |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | Liquid nitrogen immediately upon delivery |
| Transducer | Gi |
| Application | Functional assay for CCR5 |
| Safety Precautions |
Wear lab coat, disposable gloves to avoid direct sample contact. For spills: apply disinfectant, allow full inactivation, then clean up. |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
| Target Class | GPCR |
| Family | C-C motif chemokine receptors |
| Sub Family | Class A（Rhodopsin） |
| Gene Name | CCR5 |
| Gene Aliases | CKR-5;CC-CKR-5;CKR5;CD195;IDDM22 |
| Gene ID | 1234 |
| Accession Number | NM_001394783 |
| UniProt Number | P51681 |
| Protein Name | C-C chemokine receptor type 5 |
| Protein Aliases | C-C CKR-5; CC-CKR-5; CCR-5; CCR5 |
| Target Species | Human |
| Host cell | CHO-K1 |
Figure 1.Recombinant CCR5/CHO-K1 stably expressing CCR5
Blue curve: CCR5/CHO-K1 cells stained with anti-CCR5 antibody, Mean FL4-A = 2.66E6. Red curve: Parental CHO-K1 negative control cells stained with anti-CCR5 antibody, Mean FL4-A = 1892. Significant fluorescence shift confirms high membrane expression of human CCR5 receptor
![HTRF cAMP dose-response curve of recombinant human CCL4 in CCR5 CHO (C4) cells, EC50 = 2.37 nM, HTRF Ratio plotted against log[rhCCL4] concentration.](/UpLoad/Images/202608/bnl7hlz02txc.jpg)
Figure 2. HTRF cAMP Assay with CCR5 CHO-K1(C4)
Serial concentrations of recombinant human CCL4 activate CCR5-Gi signaling to modulate intracellular cAMP. The dose-response curve shows EC₅₀ = 2.37 nM, confirming robust receptor activity. This cell line is validated for CCR5 functional evaluation and high-throughput screening.
Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed CHO-K1 Human CCR5 Cell Line complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.
Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.
2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.
Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.