Committed to providing comprehensive support for global drug development

CLEC5A (C-type lectin domain family 5 member A) is a gene encoding a type II transmembrane glycoprotein located on human chromosome 12 (12q24.31). A member of the C-type lectin receptor family, it is widely expressed in myeloid cells (macrophages and neutrophils), endothelial cells, and tumor cells. Functioning as a pattern recognition receptor, it synergizes with TLR2 to activate innate immunity and promote the formation of neutrophil extracellular traps (NETs) to clear bacteria (such as *Listeria*) and viruses (such as Dengue and H5N1). However, its overactivation can trigger a cytokine storm, leading to fatal inflammatory responses—including sepsis and Dengue shock syndrome—and is associated with excessive inflammatory damage in conditions such as lupus erythematosus and inflammatory bowel disease. In the context of cancer, CLEC5A exhibits dual roles: in leukemia, gastric cancer, and glioma, it drives tumor progression by activating the AKT/mTOR pathway to promote proliferation and inhibit apoptosis; conversely, in osteosarcoma, it acts as a tumor suppressor by inhibiting the calcineurin/NFATc1 pathway, thereby reducing metastasis.

Figure 1. Recombinant CLEC5A Effector Reporter Cell(Suspension) stably expressing CLEC5A.


Figure 2. Dose Response of CLEC5A Agonist Antibodies in CLEC5A Effector Reporter Cell(Suspension,C13).

Cell Passage Procedures

1.This cell line grows in suspension.
2.Upon receipt, cells should be thawed immediately or stored in liquid nitrogen until use.
3.Before thawing, pre-warm the water bath and culture medium to 37 °C, and prepare a small amount of dry ice.
4.Remove the cryovial from storage and transport it to the cell culture laboratory on dry ice.
5.Rapidly thaw the cells in a 37 °C water bath. Once the cells are completely thawed, spray the cryovial with 70% ethanol for disinfection and transfer it to a biosafety cabinet.
6.Add 10 mL of pre-warmed culture medium into a 15 mL centrifuge tube. Transfer the contents of the cryovial into the tube and centrifuge at 1000 rpm for 5 minutes.
7.Carefully discard the supernatant. Resuspend the cell pellet in 5 mL of pre-warmed culture medium by gentle pipetting. Immediately perform cell counting and adjust the cell density to 3–6 × 10⁵ cells/mL based on the counting results, then transfer the cells into a culture flask.
8.Count the cells every 1–2 days. When the cell density exceeds 1 × 10⁶ cells/mL, passage the cells promptly or add fresh culture medium. Maintain the cell density between 2 × 10⁵ and 1 × 10⁶ cells/mL.

Suspension Cell Cryopreservation Procedure:

1.Collect 8 × 10⁶ cells, centrifuge, and discard the supernatant.
2.Add 1 mL of cell freezing medium (90% FBS + 10% DMSO) and gently pipette to mix thoroughly. Transfer the suspension into a cryovial.
3.Immediately place the cryovial into a controlled-rate freezing container (Nalgene 5100-0001), fill with isopropanol up to the indicated level, and store at −80 °C.
4.After 24 hours, transfer the cryovial to liquid nitrogen for long-term storage.