Jurkat E6.1 Human PD1 BTLA Dual-Target Effector Reporter Cell
Cat. No： RQP74682
Size： 1 vial of frozen cells (>1E6 per vial in 1 mL)
Unit Price： Contact For Pricing
Product Info
Biological Information
Assay Data
Cell Culture
| Cat. No | RQP74682 |
| Product Name | Jurkat E6.1 Human PD1 BTLA Dual-Target Effector Reporter Cell |
| Product Type | Reporter Cell |
| Culture Properties | suspension |
| Stability | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Mycoplasma Status | Negative |
| Culture Medium | RPMI-1640 + 10%FBS + 5 μg/ml Blasticidin + 1 μg/ml Puromycin + 400 μg/ml Hygromycin B |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | Liquid nitrogen immediately upon delivery |
| Application | Functional(Report Gene) Assay |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
| Classification | Combination |
| Family-1 | CD28 family |
| Gene Name-1 | PDCD1 |
| Gene Aliases-1 | CD279;PD1;hSLE1;PD-1 |
| Gene ID-1 | 5133 |
| Accession Number-1 | NM_005018 |
| UniProt Number-1 | Q15116 |
| Protein Name-1 | Protein PD-1;hPD-1 |
| Protein Aliases-1 | N/A |
| Family-2 | Immunoglobulin superfamily (IgSF) |
| Gene Name-2 | BTLA |
| Gene Aliases-2 | BTLA1;CD272; |
| Gene ID-2 | 151888 |
| Accession Number-2 | NM_181780.4 |
| UniProt Number-2 | Q7Z6A9 |
| Protein Name-2 | B- and T-lymphocyte attenuator |
| Protein Aliases-2 | B- and T-lymphocyte-associated protein |
| Target Species | Human |
| Host cell | Jurkat E6.1 |
Figure 1. Recombinant PD-1&BTLA Dual Effector Reporter Cell constitutively expressing PD-1.
Figure 2. Recombinant PD-1&BTLA Dual Effector Reporter Cell constitutively expressing BTLA.
Figure 3. Dose Response of Blocking Antibodies in PD-1&BTLA Dual Effector Reporter Cell(C59) with PD-L1&HVEM Dual aAPC Cell(C18).
Cell Passage Procedures
1.This cell line grows in suspension.
2.Upon receipt, cells should be thawed immediately or stored in liquid nitrogen until use.
3.Before thawing, pre-warm the water bath and culture medium to 37 °C, and prepare a small amount of dry ice.
4.Remove the cryovial from storage and transport it to the cell culture laboratory on dry ice.
5.Rapidly thaw the cells in a 37 °C water bath. Once the cells are completely thawed, spray the cryovial with 70% ethanol for disinfection and transfer it to a biosafety cabinet.
6.Add 10 mL of pre-warmed culture medium into a 15 mL centrifuge tube. Transfer the contents of the cryovial into the tube and centrifuge at 1000 rpm for 5 minutes.
7.Carefully discard the supernatant. Resuspend the cell pellet in 5 mL of pre-warmed culture medium by gentle pipetting. Immediately perform cell counting and adjust the cell density to 3–6 × 10⁵ cells/mL based on the counting results, then transfer the cells into a culture flask.
8.Count the cells every 1–2 days. When the cell density exceeds 1 × 10⁶ cells/mL, passage the cells promptly or add fresh culture medium. Maintain the cell density between 2 × 10⁵ and 1 × 10⁶ cells/mL.
Suspension Cell Cryopreservation Procedure:
1.Collect 8 × 10⁶ cells, centrifuge, and discard the supernatant.
2.Add 1 mL of cell freezing medium (90% FBS + 10% DMSO) and gently pipette to mix thoroughly. Transfer the suspension into a cryovial.
3.Immediately place the cryovial into a controlled-rate freezing container (Nalgene 5100-0001), fill with isopropanol up to the indicated level, and store at −80 °C.
4.After 24 hours, transfer the cryovial to liquid nitrogen for long-term storage.
info@reqbio.com
