CHO-K1 Human CCR10 Cell Line
Cat. No： RQP71355
Size： 1 vial of frozen cells (>1E6 per vial in 1 mL)
Unit Price： Contact For Pricing
Description
Product Info
Biological Info
Assay Data
Cell Culture
This product is a CHO-K1 stable cell line that stably expresses tag-free human CCR10 receptor. Upon agonist binding to CCR10, the Gi-type G protein signaling pathway is activated, inhibiting adenylyl cyclase and triggering a decrease in intracellular cAMP concentration. Therefore, receptor activity can be easily quantified in live cells in a dose-response manner by measuring changes in cAMP levels. This cell line is suitable for HTS and receptor functional evaluation assays.
Figure 1. Schematic diagram of the CCR10 CHO-K1 cell model
Assay Principle
Cells are stimulated with test compounds to modulate intracellular cAMP levels. The resulting cAMP changes are then detected through a competitive immunoassay format, and receptor activity is quantified by dose-response analysis.
| Cat. No | RQP71355 |
| Product Name | CHO-K1 Human CCR10 Cell Line |
| Engineering method | plasmid transfection |
| Culture Properties | Adherent |
| Stability | 32passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Mycoplasma Status | Negative |
| Culture Medium | F12K+10%FBS+5μg/ml puromycin |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | Liquid nitrogen immediately upon delivery |
| Transducer | Gi |
| Application | Functional assay for CCR10 |
| Safety Precautions |
Wear lab coat, disposable gloves to avoid direct sample contact. For spills: apply disinfectant, allow full inactivation, then clean up. Seal samples properly during transportation to prevent leakage and biological contamination. |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
| Target Class | GPCR |
| Family | Chemokine receptors |
| Sub Family | Class A (Rhodopsin) |
| Gene Name | CCR10 |
| Gene Aliases | GPR2 |
| Gene ID | 2826 |
| Accession Number | NM_001293 |
| UniProt Number | P46092 |
| Protein Name | C-C motif chemokine receptor 10 |
| Protein Aliases | C-C chemokine receptor type 10；CCR-10；G protein-coupled receptor 2 |
| Target Species | Human |
| Host cell | CHO-K1 |
Figure 1. Recombinant CCR10/CHO-K1 constitutively expressing CCR10
The CCR10/CHO-K1 cells (blue curve) showed high fluorescence intensity with Mean FL4-A = 2.55E5, while parental CHO-K1 cells (red curve) exhibited negligible signal (Mean FL4-A = 663). The result confirms high and stable expression of human CCR10 receptor on cell membrane.
Figure 2. HTRF cAMP Assay with CCR10 CHO-K1(C5)
CCL27 induced a dose-dependent response with EC50 = 23 nM, verifying specific receptor activity of CCR10, suitable for compound screening and pharmacological characterization.
Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed CHO-K1 Human CCR10 Cell Line complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.
Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.
2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.
Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.
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