CHO-K1 Human ADORA1 Gα15 Cell Line
Cat. No： RQP71015
Size： 1 vial of frozen cells (>1E6 per vial in 1 mL)
Unit Price： Contact For Pricing
Description
Product Info
Biological Info
Assay Data
Cell Culture
This product is a CHO-K1 stable cell line expressing tag-free full-length human ADORA1 and promiscuous Gα15. Adenosine binding to ADORA1 activates Gi to lower cAMP, while Gα15 mediates PLCβ-dependent IP1 production and calcium mobilization. Receptor activity can be quantified via cAMP, IP1 or calcium flux assays. Functionally validated with agonists, this cell line is suitable for HTS and receptor functional evaluation.
Figure 1. Schematic diagram of the ADORA1 Gα15 CHO-K1 cell model
Assay Principle
This schematic shows the IP1 time-resolved fluorescence assay for Gq GPCR detection. Agonist activates Gq signaling to raise cellular IP1. LiCl inhibits IP1 breakdown. Cellular IP1 competes with labeled tracer for anti‑IP1 antibody binding. Time-resolved fluorescence signals are measured to build dose-response curves and quantify receptor activity.
| Cat. No | RQP71015 |
| Product Name | CHO-K1 Human ADORA1 Gα15 Cell Line |
| Culture Properties | Adherent |
| Stability | 32passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Mycoplasma Status | Negative |
| Culture Medium | F12K+10% FBS+200 μg/ml Zeocin+200 μg/ml Hygromycin B |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | Liquid nitrogen immediately upon delivery |
| Transducer | Gi |
| Application | Functional assay for A1 receptor |
| Safety Precautions |
Wear lab coat, disposable gloves to avoid direct sample contact. For spills: apply disinfectant, allow full inactivation, then clean up. |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
| Target Class | GPCR |
| Family | Adenosine |
| Sub Family | Class A（Rhodopsin） |
| Gene Name | ADORA1 |
| Gene Aliases | RDC7, A1R, A1AR, AA1R, ARA1, A1-AR, Ri |
| Gene ID | 134 |
| Accession Number | NM_000674 |
| UniProt Number | P30542 |
| Protein Name | Adenosine receptor A1 |
| Protein Aliases | A1R, A1AR, AA1R, Adenosine A1 receptor |
| Target Species | Human |
| Host cell | CHO-K1 |
Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed CHO-K1 Human 5-HTR2C(no edited) Receptor Cell complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.
Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.
2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.
Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.