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TNFR2 (Tumor Necrosis Factor Receptor 2) is one of the specific receptors for TNFα and belongs to the TNFR superfamily. Primarily by activating signaling pathways such as NF-κB, it mediates cellular survival, proliferation, and immune regulatory functions; consequently, it serves as a critical therapeutic target in fields such as autoimmune diseases and tumor immunotherapy.

The TNFR2 (TNFR1 KO) Effector Reporter Cell line is a genetically engineered reporter cell line designed for the specific detection of activity within the TNFα-TNFR2 signaling pathway. Its core design involves knocking out the TNFR1 gene in effector cells and introducing a reporter gene responsive to TNFR2 signaling; this creates a pure, highly sensitive platform for investigating the biological effects mediated by TNFα via TNFR2 (rather than TNFR1), as well as for drug screening and the functional evaluation of antibodies.

The Jurkat E6.1 Human TNFR2(TNFR1 KO)  Effector Reporter Cell model accurately simulates the in vivo TNFR2 signal transduction process.

Figure 1. Sanger of TNFR2( TNFR1 KO)  Effector Reporter Cell.

Figure 2. Recombinant TNFR2( TNFR1 KO)  Effector Reporter Cell stably expressing TNFR2.

Figure 3. Dose Response of TNFR2 Agonist Ab in TNFR2(TNFR1 KO) Effector Reporter Cell(C64).


Figure 4. Dose Response of TNFR2 Agonist Ab in TNFR2(TNFR1 KO) Effector Reporter Cell(C64).


Figure 5. Blocking of TNFα induced TNFR2( TNFR1 KO) Effector Reporter Cell(C64) Activity by TNFα Neutralization Abs with Membrane Anchored TNFα Cell(Adherent, C12).


Figure 6. Blocking of TNFα induced TNFR2(TNFR1 KO) Effector Reporter Cell( C64) Activity by TNFR2 Blocking Ab with Membrane Anchored TNFα Cell ( Adherent, C12).

Cell Passage Procedures

1.This cell line grows in suspension.
2.Upon receipt, cells should be thawed immediately or stored in liquid nitrogen until use.
3.Before thawing, pre-warm the water bath and culture medium to 37 °C, and prepare a small amount of dry ice.
4.Remove the cryovial from storage and transport it to the cell culture laboratory on dry ice.
5.Rapidly thaw the cells in a 37 °C water bath. Once the cells are completely thawed, spray the cryovial with 70% ethanol for disinfection and transfer it to a biosafety cabinet.
6.Add 10 mL of pre-warmed culture medium into a 15 mL centrifuge tube. Transfer the contents of the cryovial into the tube and centrifuge at 1000 rpm for 5 minutes.
7.Carefully discard the supernatant. Resuspend the cell pellet in 5 mL of pre-warmed culture medium by gentle pipetting. Immediately perform cell counting and adjust the cell density to 3–6 × 10⁵ cells/mL based on the counting results, then transfer the cells into a culture flask.
8.Count the cells every 1–2 days. When the cell density exceeds 1 × 10⁶ cells/mL, passage the cells promptly or add fresh culture medium. Maintain the cell density between 2 × 10⁵ and 1 × 10⁶ cells/mL.

Suspension Cell Cryopreservation Procedure:

1.Collect 8 × 10⁶ cells, centrifuge, and discard the supernatant.
2.Add 1 mL of cell freezing medium (90% FBS + 10% DMSO) and gently pipette to mix thoroughly. Transfer the suspension into a cryovial.
3.Immediately place the cryovial into a controlled-rate freezing container (Nalgene 5100-0001), fill with isopropanol up to the indicated level, and store at −80 °C.
4.After 24 hours, transfer the cryovial to liquid nitrogen for long-term storage.