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Figure 1. Detect Luciferase assay by Promega Bright-Glo Luciferase Assay System.

Figure 2. Stablity test results. Raji-Luc cells showed at least 32 passage stability with similar luminescence unit.

Subculturing procedure
1. This cell is a suspension cell.
2. Upon receipt, either resuscitate the cells immediately or store them in liquid nitrogen.
3. Prior to resuscitation, preheat a water bath and culture medium to 37°C, and prepare a small amount of dry ice.
4. Retrieve the cryovial containing the cells and transport it to the cell culture room inside the dry ice.
5. Rapidly thaw the cells in the 37°C water bath; once completely thawed, spray the cryovial with sterile alcohol to disinfect it, then place it on a sterile laminar flow hood.
6. Transfer 10 mL of preheated culture medium into a 15 mL centrifuge tube; transfer the cells from the cryovial into the centrifuge tube, then centrifuge at 1000 rpm for 5 minutes.
7. Discard the supernatant, then resuspend the cell pellet by gently pipetting with 5 mL of preheated culture medium. Perform an immediate cell count; based on the results, adjust the cell density to 3–6 × 10⁵ cells/mL and transfer the suspension into a culture flask.
8. Sample the cell suspension and perform a cell count every 1–2 days. When the cell density exceeds 1 × 10⁶ cells/mL, perform a timely subculture or add fresh culture medium. Maintain the cell density within the range of 2 × 10⁵ to 1 × 10⁶ cells/mL.

Suspension Cell Cryopreservation Protocol:
1. Centrifuge 8 × 10⁶ cells and discard the supernatant.
2. Add 1 mL of cell cryopreservation medium (90% FBS + 10% DMSO), gently pipette to ensure homogeneity, and transfer the mixture into a cryovial.
3. Immediately place the vial into a cell freezing container (e.g., Nalgene 5100-0001), fill the container with isopropyl alcohol up to the indicated fill line, and transfer it to a -80°C freezer.
4. After 24 hours, transfer the cryovial to liquid nitrogen for long-term storage.