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Peptidoglycan Recognition Protein 1 (PGRP-S), also known as TAG7, is an innate immune-activating protein found in insects and mammals that mediates antibacterial activity and innate immune responses. The PGLYRP1 protein is highly expressed in immune cells—such as neutrophils, macrophages, and eosinophils—and is associated with various inflammatory conditions, including rheumatoid arthritis, airway inflammation, coronary artery disease, asthma, oral inflammation, and cancer.

The CHO-K1 Human PGLYRP1 Target Cell Model—effectively simulates the signal transduction process of TREM1 *in vivo*. The underlying principle is illustrated in the figure below.

Figure 1. Schematic Diagram of the CHO-K1 Human PGLYRP1 Target Cell Model

Figure 2. Blocking of PGLYRP1 (plus 1μg/ml PGN) Induced TREM1 Effector Reporter Cell(C33) Activity by TREM1 Blokcing Ab with PGLYRP1 Target Cell .

Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed  CHO-K1 Human PGLYRP1 Target Cell complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.

Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.

2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.

Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.