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Programmed Cell Death Protein 1 (PD-1), a receptor expressed on activated T cells, binds to its ligands PD-L1 and PD-L2 to negatively regulate immune responses. PD-1 ligands are present in most cancers, and the PD-1:PD-L1/2 interaction inhibits T cell activity, allowing cancer cells to evade immune surveillance. The PD1/PDL1 signaling pathway is an important component of tumor immune suppression, as it can inhibit T lymphocyte activation, enhance tumor cell immune tolerance, and thereby enable tumor immune escape.

Raji Human PDL1 MHCII APC Cell stably express human PDL1 based on Raji .

Figure 1. Recombinant PDL1/MHCII APC Cell constitutively expressing PDL1.

Figure 2. Dose Response of Blocking Antibodies in PD-1/LAG3 Dual Effector Reporter Cells (C15) with PD-L1/MHCII APC Cells.

Cell Passage Procedures

1.This cell line grows in suspension.
2.Upon receipt, cells should be thawed immediately or stored in liquid nitrogen until use.
3.Before thawing, pre-warm the water bath and culture medium to 37 °C, and prepare a small amount of dry ice.
4.Remove the cryovial from storage and transport it to the cell culture laboratory on dry ice.
5.Rapidly thaw the cells in a 37 °C water bath. Once the cells are completely thawed, spray the cryovial with 70% ethanol for disinfection and transfer it to a biosafety cabinet.
6.Add 10 mL of pre-warmed culture medium into a 15 mL centrifuge tube. Transfer the contents of the cryovial into the tube and centrifuge at 1000 rpm for 5 minutes.
7.Carefully discard the supernatant. Resuspend the cell pellet in 5 mL of pre-warmed culture medium by gentle pipetting. Immediately perform cell counting and adjust the cell density to 3–6 × 10⁵ cells/mL based on the counting results, then transfer the cells into a culture flask.
8.Count the cells every 1–2 days. When the cell density exceeds 1 × 10⁶ cells/mL, passage the cells promptly or add fresh culture medium. Maintain the cell density between 2 × 10⁵ and 1 × 10⁶ cells/mL.

Suspension Cell Cryopreservation Procedure:

1.Collect 8 × 10⁶ cells, centrifuge, and discard the supernatant.
2.Add 1 mL of cell freezing medium (90% FBS + 10% DMSO) and gently pipette to mix thoroughly. Transfer the suspension into a cryovial.
3.Immediately place the cryovial into a controlled-rate freezing container (Nalgene 5100-0001), fill with isopropanol up to the indicated level, and store at −80 °C.
4.After 24 hours, transfer the cryovial to liquid nitrogen for long-term storage.