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Transmembrane Activator and CAML Interactor (TACI) is a member of the Tumor Necrosis Factor Receptor (TNFR) superfamily, also known as TNFRSF13B. TACI acts as a regulator of immune responses, inhibiting B cell proliferation while promoting plasma cell differentiation and survival.

The ligands for TACI are BAFF and APRIL. BAFF is a type II transmembrane protein belonging to the TNF ligand superfamily; it exists in two forms: a membrane-bound form and a soluble trimeric form. APRIL also belongs to the TNF ligand superfamily and is a type II transmembrane protein involved in late-stage B cell differentiation, the generation and maintenance of antibody-secreting plasma cells, and IgA class switching. Myeloid cells (including macrophages, dendritic cells, and circulating monocytes), as well as polymorphonuclear cells (such as neutrophils, eosinophils, and mucosal epithelial cells), are responsible for the production of APRIL. APRIL exhibits a strong binding affinity for BCMA and a moderate affinity for TACI, whereas BAFF binds weakly to BCMA but demonstrates a stronger affinity for TACI. TACI interacts with both APRIL and Heparan Sulfate Proteoglycans (HSPGs), thereby facilitating efficient TACI signal transduction to exert its physiological effects.

The TACI Effector Reporter Cell drug target model accurately simulates the in vivo signal transduction processes of TACI; the underlying principle is illustrated in the figure below.

Figure 1. Schematic Diagram of the Jurkat E6.1 Human TACI Effector Reporter Cell Model

Figure 2. Recombinant TACI Effector Reporter Cell stably expressing TACI.

Figure 3. Dose response of Ligands in TACI Effector Reporter Cell(C32).

Figure 4. Inhibition of hBAFF¬induced Reporter Activity by BAFF Neutralization in TACI Effector Reporter Cell(C32).Inhibition of hAPRIL-induced Reporter Activity by APRIL Neutralization in TACI Effector Reporter Cell(C32).

Figure 5. Blocking of BAFF induced TACI Effector Reporter Cell(C32) Activity by BAFF Neutralization Ab with BAFF CHO.

Cell Passage Procedures

1.This cell line grows in suspension.
2.Upon receipt, cells should be thawed immediately or stored in liquid nitrogen until use.
3.Before thawing, pre-warm the water bath and culture medium to 37 °C, and prepare a small amount of dry ice.
4.Remove the cryovial from storage and transport it to the cell culture laboratory on dry ice.
5.Rapidly thaw the cells in a 37 °C water bath. Once the cells are completely thawed, spray the cryovial with 70% ethanol for disinfection and transfer it to a biosafety cabinet.
6.Add 10 mL of pre-warmed culture medium into a 15 mL centrifuge tube. Transfer the contents of the cryovial into the tube and centrifuge at 1000 rpm for 5 minutes.
7.Carefully discard the supernatant. Resuspend the cell pellet in 5 mL of pre-warmed culture medium by gentle pipetting. Immediately perform cell counting and adjust the cell density to 3–6 × 10⁵ cells/mL based on the counting results, then transfer the cells into a culture flask.
8.Count the cells every 1–2 days. When the cell density exceeds 1 × 10⁶ cells/mL, passage the cells promptly or add fresh culture medium. Maintain the cell density between 2 × 10⁵ and 1 × 10⁶ cells/mL.

Suspension Cell Cryopreservation Procedure:

1.Collect 8 × 10⁶ cells, centrifuge, and discard the supernatant.
2.Add 1 mL of cell freezing medium (90% FBS + 10% DMSO) and gently pipette to mix thoroughly. Transfer the suspension into a cryovial.
3.Immediately place the cryovial into a controlled-rate freezing container (Nalgene 5100-0001), fill with isopropanol up to the indicated level, and store at −80 °C.
4.After 24 hours, transfer the cryovial to liquid nitrogen for long-term storage.