Committed to providing comprehensive support for global drug development

The Lymphotoxin-beta receptor (LTBR), also known as Tumor Necrosis Factor Receptor Superfamily Member 3 (TNFRSF3), is a cell-surface receptor involved in apoptosis and cytokine release. LTBR is a type I single-pass transmembrane protein and a member of the Tumor Necrosis Factor Receptor (TNFR) family; it plays a pivotal role in the development of secondary lymphoid organs. LTBR is expressed by a variety of immune cells—including lymphoid tissue stromal cells, myeloid cells, monocytes, alveolar macrophages, mast cells, dendritic cells (DCs), and most other immune cells—but is not expressed in T cells, B cells, or NK cells.

LTBR has two distinct natural ligands: LTα1β2 and LIGHT (TNFSF14). LTα1β2 does not bind to any molecule other than LTBR, ​​whereas LIGHT also binds to HVEM.

The Jurkat E6.1 Human LTBR Effector Reporter Cell Model—effectively simulates the signal transduction process of LTBR *in vivo*. The underlying principle is illustrated in the figure below.

Figure 1. Schematic Diagram of the Jurkat E6.1 Human LTBR Effector Reporter Cell Model

Figure 2. Recombinant LTBR Effector Reporter Cell stably expressing LTBR.

Figure 3. Dose Response of  Human LTBR Agonist Antibody in LTBR Effector Reporter Cell (C68).

Cell Passage Procedures

1.This cell line grows in suspension.
2.Upon receipt, cells should be thawed immediately or stored in liquid nitrogen until use.
3.Before thawing, pre-warm the water bath and culture medium to 37 °C, and prepare a small amount of dry ice.
4.Remove the cryovial from storage and transport it to the cell culture laboratory on dry ice.
5.Rapidly thaw the cells in a 37 °C water bath. Once the cells are completely thawed, spray the cryovial with 70% ethanol for disinfection and transfer it to a biosafety cabinet.
6.Add 10 mL of pre-warmed culture medium into a 15 mL centrifuge tube. Transfer the contents of the cryovial into the tube and centrifuge at 1000 rpm for 5 minutes.
7.Carefully discard the supernatant. Resuspend the cell pellet in 5 mL of pre-warmed culture medium by gentle pipetting. Immediately perform cell counting and adjust the cell density to 3–6 × 10⁵ cells/mL based on the counting results, then transfer the cells into a culture flask.
8.Count the cells every 1–2 days. When the cell density exceeds 1 × 10⁶ cells/mL, passage the cells promptly or add fresh culture medium. Maintain the cell density between 2 × 10⁵ and 1 × 10⁶ cells/mL.

Suspension Cell Cryopreservation Procedure:

1.Collect 8 × 10⁶ cells, centrifuge, and discard the supernatant.
2.Add 1 mL of cell freezing medium (90% FBS + 10% DMSO) and gently pipette to mix thoroughly. Transfer the suspension into a cryovial.
3.Immediately place the cryovial into a controlled-rate freezing container (Nalgene 5100-0001), fill with isopropanol up to the indicated level, and store at −80 °C.
4.After 24 hours, transfer the cryovial to liquid nitrogen for long-term storage.