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c-Met belongs to the receptor tyrosine kinase family; it is a heterodimer composed of a highly glycosylated 50 kDa extracellular α-chain and a 140 kDa transmembrane β-chain, linked together by disulfide bonds. As the natural receptor for HGF, it is expressed on the surface of various epithelial cells and promotes tumorigenesis. Upon binding with HGF, the two subunits dimerize, leading to the autophosphorylation of two tyrosine residues (Tyr1234 and Tyr1235). This autophosphorylation of tyrosine residues generates an activated multifunctional docking site—comprising an SH2 domain, a PTB domain, and a Met-binding domain (MDB)—which serves to recruit adaptor molecules.

The MET Dimerization CHO Reporter Gene Drug Target Model accurately simulates the in vivo signal transduction process of MET, as illustrated in the diagram below.

Figure 1. Schematic Diagram of the CHO-K1 Human MET Dimerization Cell Model

Figure 2. Recombinant MET Dimerization CHO stably expressing MET.

Figure 3. Dose Response of Recombinant Human HGF in Met Dimerization CHO(C19).

Figure 4. Inhibition of Recombinant Human HGF induced Met Dimerization by Met Abs in Met Dimerization CHO(C19).

Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed  CHO-K1 Human MET Dimerization Cell complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.

Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.

2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.

Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.