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The IL15RA gene is located on chromosome 10p15.1 and encodes the Interleukin-15 receptor alpha chain (CD215), which belongs to the Type I cytokine receptor family. Its structure comprises a 173-amino-acid extracellular domain (containing a Sushi domain), a 21-amino-acid transmembrane domain, and a 37-amino-acid cytoplasmic domain. It binds to IL-15 with high affinity to form a heterotrimeric receptor complex (associating with the IL-2Rβ and γc subunits), thereby activating signaling pathways such as JAK/STAT, PI3K/AKT, and MAPK. This activation promotes the proliferation and survival of T cells and NK cells, and maintains the homeostasis of memory CD8+ T cells.

Functionally, IL15RA participates in regulating the homeostasis of memory CD8+ T cells and NK cells, and enhances anti-tumor immune responses. It also plays a role in muscular endurance; mice in which this gene has been knocked out exhibit superior exercise endurance. Furthermore, aberrant expression of IL15RA is associated with autoimmune diseases (such as rheumatoid arthritis), cancer, and infectious diseases—specifically, HIV and HTLV-1 infections.

IL15RA CHO-K1 Cells: A cell line derived from CHO-K1 cells that stably expresses human IL15RA.

Figure 1. Recombinant IL15RA CHO stably expressing IL15RA.

Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed  CHO-K1 Human IL15RA Cell complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.

Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.

2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.

Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.