CHO-K1 Human EDG1(S1P1) β-Arrestin Cell Line
Cat. No： RQP71554
Size： 1 vial of frozen cells (>1E6 per vial in 1 mL)
Unit Price： Contact For Pricing
Description
Product Info
Biological Information
Assay Data
Cell Culture
This product is a CHO-K1 stable cell line that stably expresses human EDG1 (S1P1) receptor for β‑arrestin recruitment assay. Upon agonist binding to EDG1 (S1P1), β‑arrestin is recruited to the activated receptor, reconstituting split luciferase and generating luminescent signal after substrate addition. Therefore, receptor activity can be easily quantified in live cells in a dose-response manner by measuring luminescence intensity. This cell line is suitable for high-throughput screening (HTS) and receptor functional evaluation assays.
| Cat. No | RQP71554 |
| Product Name | CHO-K1 Human EDG1(S1P1) β-Arrestin Cell Line |
| Engineering method | plasmid and lentivirus |
| Culture Properties | Adherent |
| Stability | 32passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Mycoplasma Status | Negative |
| Culture Medium | F12K+10%FBS+5 μg/ml Puromycin+5 μg/ml Blasticidin |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | Liquid nitrogen immediately upon delivery |
| Transducer | β-Arrestin |
| Application | Functional assay for EDG1(S1P1) receptor |
| Safety Precautions |
Wear lab coat, disposable gloves to avoid direct sample contact. For spills: apply disinfectant, allow full inactivation, then clean up. |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
| Target Class | GPCR |
| Family | Sphingosine 1-phosphate receptors |
| Sub Family | Class A（Rhodopsin） |
| Gene Name | EDG1 |
| Gene Aliases | S1PR1;edg-1;D1S3362;CD363 |
| Gene ID | 1901 |
| Accession Number | NM_001400 |
| UniProt Number | P21453 |
| Protein Name | S1P receptor 1; S1P1 |
| Protein Aliases | Endothelial differentiation G-protein coupled receptor 1; Sphingosine 1-phosphate receptor Edg-1 (S1P receptor Edg-1) |
| Target Species | Human |
| Host cell | CHO-K1 |
Figure 1. Dose Response of S1P in EDG1(S1P1) β-Arrestin CHO-K1(C6)
S1P induces a robust, concentration-dependent β-arrestin recruitment signal. The EC₅₀ value is 30.2 nM, indicating potent agonist activity of S1P toward EDG1(S1P1).
Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed CHO-K1 Human EDG1(S1P1) β-Arrestin Cell Line complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.
Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.
2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.
Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.