CHO-K1 Human CB1 β-Arrestin Cell Line
Cat. No： RQP71391
Size： 1 vial of frozen cells (>1E6 per vial in 1 mL)
Unit Price： Contact For Pricing
Description
Product Info
Biological Info
Assay Data
Cell Culture
This product is a CHO-K1 stable cell line that stably expresses tag-free full-length human CB1 receptor together with a β-arrestin–luciferase reporter system. Upon agonist binding to human CB1, β-arrestin is recruited to the activated receptor, driving split-luciferase complementation and generating a quantifiable luminescent signal upon substrate addition. Therefore, receptor activity can be easily quantified in live cells in a dose-response manner by measuring the luminescent signal. The cell line has been functionally validated with positive agonists. It is suitable for high-throughput screening (HTS) and receptor functional evaluation assays.
Figure 1. Schematic diagram of CB1 β-Arrestin CHO-K1 cell model
Assay Principle
Upon agonist binding, the GPCR becomes activated and recruits β-arrestin. The β-arrestin-luciferase fusion protein is brought into proximity with the receptor complex. Addition of substrate restores luciferase activity and generates luminescence signals. The luminescence intensity correlates with the level of β-arrestin recruitment, which can be quantified via a plate reader to generate dose-response curves for ligand characterization.
| Cat. No | RQP71391 |
| Product Name | CHO-K1 Human CB1 β-Arrestin Cell Line |
| Engineering method | plasmid and lentivirus |
| Culture Properties | Adherent |
| Stability | 32passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Mycoplasma Status | Negative |
| Culture Medium | F12k+10%FBS+5μg/ml puromycin+5μg/ml blasticidin |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | Liquid nitrogen immediately upon delivery |
| Transducer | β-Arrestin |
| Application | Functional assay for CNR1 |
| Safety Precautions |
Wear lab coat, disposable gloves to avoid direct sample contact. For spills: apply disinfectant, allow full inactivation, then clean up. Seal samples properly during transportation to prevent leakage and biological contamination. |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
| Target Class | GPCR |
| Family | Cannabinoid receptors |
| Sub Family | Class A（Rhodopsin） |
| Gene Name | CNR1 |
| Gene Aliases | CB1，CB1K5，CB-R，CANN6，CB1A |
| Gene ID | 1268 |
| Accession Number | NM_016083 |
| UniProt Number | P21554 |
| Protein Name | CB-R，CB1，Cannabinoid receptor 1 |
| Protein Aliases | CANN6，Cannabinoid receptor 1;CB-R |
| Target Species | Human |
| Host cell | CHO-K1 |
Figure 1. Recombinant CB1/β-Arrestin/CHO-K1 constitutively expressing CB1
The recombinant CB1/β-Arrestin/CHO-K1 cells constitutively expressing CB1 (blue curve) exhibited strong APC fluorescence signal (Mean = 3.34E5), while β-Arrestin/CHO-K1 control cells (red curve) showed negligible background signal (Mean = 482), verifying stable membrane expression of CB1 receptor.
Figure 2. Dose response of CP-55940 in CB1 β-Arrestin CHO-K1 Cell Line (C2)
Agonist CP-55940 induced a robust dose-dependent response with EC50 = 3.37 nM and signal-to-background ratio (S/B) = 57.4, confirming potent receptor activity suitable for high-throughput screening and QC lot release.
Figure 3. Inhibition of CP-55940-induced Beta-Arrestin Recruitment in CB1 Beta-Arrestin CHO-K1 Cells (C2)
Nimacimab exhibited dose-dependent inhibition of CP-55940-triggered β-arrestin recruitment, with IC50 = 0.54 μg/ml, demonstrating suitability for antagonist screening and QC lot release.
Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed CHO-K1 Human CB1 β-Arrestin Cell Line complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.
Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.
2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.
Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.