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In humans, SCF220 is located at chromosome 12q22-24, sharing the same genetic locus as soluble SCF (SCF248) and generating different isoforms through alternative mRNA splicing; in mice, it is situated at the *Steel* locus on chromosome 10, where the encoded membrane-bound SCF (mSCF220) and soluble SCF (mSCF248) jointly participate in the regulation of hematopoiesis. The membrane-bound SCF encoded by the SCF220 gene is a key factor in hematopoietic regulation, cell development, and immune modulation; its dual forms (membrane-bound and soluble) offer multi-target strategies for clinical intervention. Future research will focus on elucidating underlying mechanisms and advancing translational applications, thereby realizing its potential in cancer therapy, bone marrow transplantation, and tissue repair.

SCF220 Jurkat cells: Jurkat cells stably expressing human SCF220.

Figure 1. Recombinant SCF220 Jurkat stably expressing SCF220.

Cell Passage Procedures

1.This cell line grows in suspension.
2.Upon receipt, cells should be thawed immediately or stored in liquid nitrogen until use.
3.Before thawing, pre-warm the water bath and culture medium to 37 °C, and prepare a small amount of dry ice.
4.Remove the cryovial from storage and transport it to the cell culture laboratory on dry ice.
5.Rapidly thaw the cells in a 37 °C water bath. Once the cells are completely thawed, spray the cryovial with 70% ethanol for disinfection and transfer it to a biosafety cabinet.
6.Add 10 mL of pre-warmed culture medium into a 15 mL centrifuge tube. Transfer the contents of the cryovial into the tube and centrifuge at 1000 rpm for 5 minutes.
7.Carefully discard the supernatant. Resuspend the cell pellet in 5 mL of pre-warmed culture medium by gentle pipetting. Immediately perform cell counting and adjust the cell density to 3–6 × 10⁵ cells/mL based on the counting results, then transfer the cells into a culture flask.
8.Count the cells every 1–2 days. When the cell density exceeds 1 × 10⁶ cells/mL, passage the cells promptly or add fresh culture medium. Maintain the cell density between 2 × 10⁵ and 1 × 10⁶ cells/mL.

Suspension Cell Cryopreservation Procedure:

1.Collect 8 × 10⁶ cells, centrifuge, and discard the supernatant.
2.Add 1 mL of cell freezing medium (90% FBS + 10% DMSO) and gently pipette to mix thoroughly. Transfer the suspension into a cryovial.
3.Immediately place the cryovial into a controlled-rate freezing container (Nalgene 5100-0001), fill with isopropanol up to the indicated level, and store at −80 °C.
4.After 24 hours, transfer the cryovial to liquid nitrogen for long-term storage.