Committed to providing comprehensive support for global drug development

Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14), is a human cell surface receptor belonging to the tumor necrosis factor (TNF) receptor superfamily. HVEM is expressed on various immune cells, including T cells, B cells, natural killer cells, dendritic cells, and endothelial cells. It also contributes to tumor immune evasion through its frequent expression on melanoma cells.

HVEM has four distinct ligands—TNFSF14, LTA, BTLA, and CD160—that play key roles in signal transduction. Acting as a molecular switch, HVEM interacts with its own LIGHT family members by activating the transcription factor NF-κB, thereby increasing inflammatory responses and enhancing immune responses. It can induce signaling cascades through their death domains or TRAF-mediated pathways, leading to the activation of cell survival or apoptosis.

 The HVEM NFκB-Luc Jurkat reporter gene drug target model effectively mimics the in vivo signaling pathway of HVEM; the mechanism is illustrated in the figure below.

Figure 1. Schematic diagram of the HVEM NFκB-Luc Jurkat cell model

Figure 2. Recombinant Jurkat stably expressing HVEM.

Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). HVEM NFκB-Luc Jurkat(C7) Report Assay Stimulated by LIGHT/CHO.

Cell Passage Procedures

1.This cell line grows in suspension.
2.Upon receipt, cells should be thawed immediately or stored in liquid nitrogen until use.
3.Before thawing, pre-warm the water bath and culture medium to 37 °C, and prepare a small amount of dry ice.
4.Remove the cryovial from storage and transport it to the cell culture laboratory on dry ice.
5.Rapidly thaw the cells in a 37 °C water bath. Once the cells are completely thawed, spray the cryovial with 70% ethanol for disinfection and transfer it to a biosafety cabinet.
6.Add 10 mL of pre-warmed culture medium into a 15 mL centrifuge tube. Transfer the contents of the cryovial into the tube and centrifuge at 1000 rpm for 5 minutes.
7.Carefully discard the supernatant. Resuspend the cell pellet in 5 mL of pre-warmed culture medium by gentle pipetting. Immediately perform cell counting and adjust the cell density to 3–6 × 10⁵ cells/mL based on the counting results, then transfer the cells into a culture flask.
8.Count the cells every 1–2 days. When the cell density exceeds 1 × 10⁶ cells/mL, passage the cells promptly or add fresh culture medium. Maintain the cell density between 2 × 10⁵ and 1 × 10⁶ cells/mL.

Suspension Cell Cryopreservation Procedure:

1.Collect 8 × 10⁶ cells, centrifuge, and discard the supernatant.
2.Add 1 mL of cell freezing medium (90% FBS + 10% DMSO) and gently pipette to mix thoroughly. Transfer the suspension into a cryovial.
3.Immediately place the cryovial into a controlled-rate freezing container (Nalgene 5100-0001), fill with isopropanol up to the indicated level, and store at −80 °C.
4.After 24 hours, transfer the cryovial to liquid nitrogen for long-term storage.