Jurkat E6.1 Human ADCP Bioassay Effector Cell FcγRIIb-NFAT
Cat. No： RQP74574
Size： 1 vial of frozen cells (>1E6 per vial in 1 mL)
Unit Price： Contact For Pricing
Description
Product Info
Biological Information
Assay Data
Cell Culture
ADCP (Antibody-Dependent Cellular Phagocytosis) is an immune clearance mechanism in which monoclonal antibodies (mAbs) target tumor cells, thereby facilitating their engulfment and removal from the body by phagocytic immune cells. This process is mediated by monocytes, macrophages, neutrophils, and dendritic cells expressing FcγRIIa (CD32a), FcγRI (CD64), and FcγRIIIa (CD16a); research indicates that FcγRIIa is a key FcγR receptor involved in this process.
Traditional methods for assessing ADCC/ADCP rely heavily on the isolation and *in vitro* differentiation of relevant primary immune cells to measure the subsequent killing or phagocytosis of target cells. These methods are costly and labor-intensive, relying heavily on donor-derived primary cells. The isolation and differentiation processes demand high technical proficiency and carry a significant risk of experimental failure; furthermore, issues regarding cell purity can lead to weak, unstable, or inconsistent detection signals. Additionally, primary cells are non-renewable, and variations in donor sources across experiments can result in poor reproducibility and batch-to-batch variability. Consequently, it is difficult to establish stable, reliable assay methods suitable for drug development processes that require rigorous quality control.
The ADCP bioassay model—utilizing effector cells expressing FcγRIIb and a Jurkat-NFAT reporter system—effectively simulates the ADCP signaling transduction process *in vivo*; the underlying mechanism is illustrated in the figure below.
Figure 1. Schematic diagram of the Jurkat E6.1 Human ADCP Bioassay Effector Cell FcγRIIb-NFAT.
| Cat. No | RQP74574 |
| Product Name | Jurkat E6.1 Human ADCP Bioassay Effector Cell FcγRIIb-NFAT |
| Product Type | Reporter Cell |
| Culture Properties | suspension |
| Stability | 32passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Mycoplasma Status | Negative |
| Culture Medium | RPMI-1640+10%FBS+1 μg/ml Puromycin+400 μg/ml Hygromycin B |
| Freeze Medium | 90% FBS+10% DMSO |
| Storage Conditions | Liquid nitrogen immediately upon delivery |
| Application | Functional(Report Gene) Assay |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
| Classification | Fc Effector |
| Family | Fc receptor family (FcγR family) |
| Gene Name | FCGR2B |
| Gene Aliases | CD32, CD32B, CDw32, FCG2, IGFR2, Fc-gamma RII-b |
| Gene ID | 2213 |
| Accession Number | NM_001394477.1 |
| UniProt Number | P31994 |
| Protein Name | Low affinity immunoglobulin gamma Fc region receptor II-b |
| Protein Aliases | Fc-gamma RII-b, FcRII-b, CD32 |
| Target Species | Human |
| Host cell | Jurkat E6.1 |
Figure 2. Recombinant ADCP Bioassay Effector Cell FcγRIIb-NFAT Jurkat stably expressing FcγRIIb.
Figure 3. Dose Response of Rituximab in ADCP Bioassay Effector Cell FcγRIIb -NFAT Jurkat(C30) with Raji.
Cell Passage Procedures
1.This cell line grows in suspension.
2.Upon receipt, cells should be thawed immediately or stored in liquid nitrogen until use.
3.Before thawing, pre-warm the water bath and culture medium to 37 °C, and prepare a small amount of dry ice.
4.Remove the cryovial from storage and transport it to the cell culture laboratory on dry ice.
5.Rapidly thaw the cells in a 37 °C water bath. Once the cells are completely thawed, spray the cryovial with 70% ethanol for disinfection and transfer it to a biosafety cabinet.
6.Add 10 mL of pre-warmed culture medium into a 15 mL centrifuge tube. Transfer the contents of the cryovial into the tube and centrifuge at 1000 rpm for 5 minutes.
7.Carefully discard the supernatant. Resuspend the cell pellet in 5 mL of pre-warmed culture medium by gentle pipetting. Immediately perform cell counting and adjust the cell density to 3–6 × 10⁵ cells/mL based on the counting results, then transfer the cells into a culture flask.
8.Count the cells every 1–2 days. When the cell density exceeds 1 × 10⁶ cells/mL, passage the cells promptly or add fresh culture medium. Maintain the cell density between 2 × 10⁵ and 1 × 10⁶ cells/mL.
Suspension Cell Cryopreservation Procedure:
1.Collect 8 × 10⁶ cells, centrifuge, and discard the supernatant.
2.Add 1 mL of cell freezing medium (90% FBS + 10% DMSO) and gently pipette to mix thoroughly. Transfer the suspension into a cryovial.
3.Immediately place the cryovial into a controlled-rate freezing container (Nalgene 5100-0001), fill with isopropanol up to the indicated level, and store at −80 °C.
4.After 24 hours, transfer the cryovial to liquid nitrogen for long-term storage.
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We Are Pleased to Announce: Global Commercial Licensing Rights for Jurkat E6.1, CHO-K1, HEK293, THP-1 and RAJI Cell Lines Officially Secured.
ExploreWe Are Pleased to Announce: Global Commercial Licensing Rights for Jurkat E6.1, CHO-K1, HEK293, THP-1 and RAJI Cell Lines Officially Secured.
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