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The IL10RB gene, short for interleukin-10 receptor subunit β, is located on chromosome 21 in humans. It is 43,816 bp in length, contains 7 exons, and encodes a 325-amino acid protein. Belonging to the class II cytokine receptor family, it is an essential helper chain in the IL-10 active receptor complex. It must be expressed alone and co-expressed with IL10RA to initiate downstream IL-10 signaling.

This gene has a wide range of functions. The encoded protein is a common receptor subunit for five class II cytokines. It participates in IL-10-mediated anti-inflammatory immune regulation and, as a component of the IFNLR1/IL10RB dimer, mediates the antiviral activity of IFNL2 and IFNL3. It also activates the JAK/STAT pathway to induce interferon-stimulated gene expression, playing a crucial role in the body's antiviral defense.

Figure 1. Recombinant HEK293 Human IL10RB Cell stably expressing IL10RB.

Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed HEK293 Human IL10RB Cell complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.

Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.

2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.

Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.