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Activin E (encoded by the *INHBE* gene) and ALK7 (encoded by the *ACVR1C* gene) constitute a ligand-receptor pair within the TGF-β superfamily. Upon binding to ALK7, Activin E activates the SMAD2/3 signaling pathway, thereby strongly inhibiting lipolysis in adipocytes. This pathway promotes lipid storage while suppressing lipolysis. Consequently, blocking the Activin E/ALK7 signaling axis has emerged as a promising strategy for the treatment of obesity and associated metabolic disorders.

The HEK293 Human Activin E/ALK7 Effector Reporter Cell model accurately mimics the in vivo signal transduction process of Activin E/ALK7.

Figure 1. Dose Response of Recombinant Human Activin E in Activin E/ALK7 Effector Reporter Cell( C34).


Figure 2. Inhibition of Human Activin E Induced Reporter Activity by ALK7 Blocking Ab in ActivinE/ALK7 Effector Reporter Cell( C34).

Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed HEK293 Human Activin E/ALK7 Effector Reporter Cellcomplete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.

Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.

2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.

Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.