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Cynomolgus monkey IL-20, IL-22, and IL-24 all belong to the IL-10 cytokine family; they share IL-22Rα1 (also known as IL-22RA1) as a common receptor subunit and activate the JAK-STAT signaling pathway by forming distinct receptor complexes. Among them, IL-20 is a pro-inflammatory cytokine that plays a significant role in inflammatory diseases such as psoriasis and rheumatoid arthritis; IL-22 is primarily involved in tissue repair and mucosal immunity, promoting epithelial cell proliferation and defense against pathogen infection; and IL-24—also known as "melanoma differentiation-associated gene-7" due to its ability to induce apoptosis in tumor cells—possesses anti-angiogenic properties.

Figure 1. Dose Response of Ligands in Cyno IL20&IL22&IL24 Triple Effector Reporter Cell(C30).


Figure 2. Inhibition of hIL20 Induced Reporter Activity by IL22Ra Blocking Ab or IL20 Neutralization Ab in Cyno IL20&IL22&IL24 Triple Effector Cell(C30).



Figure 3. Inhibition of Cyno IL22 Induced Reporter Activity by IL22Ra Blocking Ab or IL22 Neutralization Ab in Cyno IL20&IL22&IL24 Triple Effector Cell(C30).



Figure 4. Inhibition of hIL24 Induced Reporter Activity by IL22Ra Blocking Ab or IL24 Neutralization Ab in Cyno IL20&IL22&IL24 Triple Effector Cell (C30).

Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed HEK293 Cynomolgus IL20&IL22&IL24 Effector Reporter Cell complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.

Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.

2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.

Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.