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 TL1A, also known as TNFSF15, is a member of the TNF superfamily and is expressed in both immune cells (such as monocytes, macrophages, dendritic cells, and T cells) and non-immune cells (such as synovial fibroblasts and endothelial cells). TL1A is a type II transmembrane protein that self-assembles into a stable trimer through interactions with TNF-homologous domains (THDs). It is primarily expressed in a membrane-bound form and forms a stable trimer. Soluble TL1A (sTL1A) is generated through alternative splicing or cleavage by TNF-α convertase (TACE).

      TL1A competitively binds to death receptor 3 (DR3) or decoy receptor 3 (DcR3), providing a stimulus for downstream signaling pathways, thereby regulating the proliferation, activation, apoptosis, and production of cytokines and chemokines in effector cells.

As the target cell for the DR3 effector reporter cell, TL1A effectively mimics the in vivo signal transduction process of DR3; the mechanism is illustrated in the figure below.

Figure 1. Schematic Diagram of the TL1A Target Cell Construction

Figure 2. Recombinant TL1A Target Cell stably expressing TL1A.


Figure 3. Blocking of TLlA induced DR3 Effector Reporter Cell(C15) Activity by TLlA Neutralizaiton Abs with TL1A Target Cell.

Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed  CHO-K1 Human TL1A Target Cell complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.

Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.

2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.

Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.