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The IL10RB gene, encoding interleukin-10 receptor subunit beta, is located on human chromosome 21; it spans 43,816 bp and comprises seven exons. The encoded protein consists of 325 amino acids and belongs to the class II cytokine receptor family. It serves as an essential accessory chain for the functional IL-10 receptor complex, and its co-expression with IL10RA is required to initiate IL-10 downstream signaling. This gene performs diverse functions: the encoded protein acts as a shared receptor subunit for five class II cytokines, participating in IL-10-mediated anti-inflammatory immune regulation. Additionally, as a component of the IFNLR1/IL10RB dimer, it mediates the antiviral activities of IFNL2 and IFNL3, activates the JAK/STAT pathway to induce the expression of interferon-stimulated genes, and plays a critical role in the host's antiviral defense.

Figure 1. Recombinant IL10RB CHO stably expressing IL10RB.

Cell Resuscitation
1)Rapidly thaw the frozen cells in a 37 °C water bath for approximately 60 seconds. Once thawed (which may take slightly less or more than 60 seconds), immediately transfer the cell suspension from the cryovial into a 15 mL centrifuge tube containing 10 mL of pre-warmed  CHO-K1 Human IL10RB Cell complete culture medium.
2)Centrifuge cells at 1000 rpm for 5 min to remove medium, then resuspend cells in 5 mL of pre-warmed complete medium.
3)Transfer the cell suspension into a T25 culture flask and incubate at 37 °C with 5% CO₂.
4)After approximately 24–36 hours, replace the medium or passage the cells to remove non-adherent dead cells.

Subculturing procedure
1)When the cell density reaches the appropriate confluency for passaging, wash the cells with PBS, then add 1 mL trypsin to detach the cells. When more than 80% of the cells detach upon gently tapping the culture flask, add complete culture medium to terminate digestion. Gently pipette to obtain a single-cell suspension, transfer to a 15 mL centrifuge tube, and centrifuge at 1000 rpm for 5 minutes.

2)Discard supernatant after centrifugation. Resuspend cells in fresh medium to a single-cell suspension and transfer to a new culture flask for continued growth.

Cell Freezing
After trypsinization and centrifugation of cells from each T75 flask or 10 cm culture dish, discard the supernatant. Add 2 mL of cryopreservation medium (90% FBS + 10% DMSO), gently resuspend thoroughly, and aliquot into two cryovials. Immediately place the cryovials into a controlled-rate freezing container (e.g., Nalgene 5100-0001), fill with isopropanol to the indicated level, and store at −80 °C. After 24 hours, transfer the cryovials to liquid nitrogen for long-term storage.