Class C glutamate receptors contain 22 receptors, divided into 5 subfamilies, including 1 calcium-sensitive receptor (CaSR), 2 GABA type B receptors (GABAB1 and GABAB2), 3 taste 1 receptors (TS1R1-3), 8 metabolic glutamate receptors (mGluR1-8), and 8 orphan GPCRs.
L-Glutamate induced a dose-dependent response with EC50 = 5.03 μM. The data confirms functional GRM4 receptor activity, suitable for pharmacological profiling and compound screening.
Figure 2. Dose-response of Etelcalcetide hydrochloride in CaSR NFAT-Luc HEK293 (C15) cells
Red curve: Etelcalcetide hydrochloride alone, EC50 = 14.4 μM. Yellow curve: Etelcalcetide hydrochloride with 1 mM CaCl₂, EC50 = 1.52 μM. The data demonstrates allosteric potentiation of CaSR, confirming functional receptor activity for pharmacological study and compound screening.
