Professional technology integration to support the entire R&D process

An sgRNA library, short for "single guide RNA library," is a collection of tens to hundreds of thousands of different sgRNA molecules. Each sgRNA acts like a "molecular key," precisely guiding the CRISPR/Cas9 gene editing system to a specific site on the genome for cutting. Therefore, an sgRNA library is essentially a vast "toolbox" that can target virtually any gene, allowing scientists to simultaneously conduct large-scale functional gene screening across the entire genome.

Investigating gene function in metabolic pathways and disease processes plays a critical role in uncovering disease mechanisms and facilitating therapeutic development. CRISPR-Cas9, as a next-generation gene editing technology, has emerged as a robust platform for forward genetic screening, owing to its simplicity, scalability, and versatility. Genome-wide or targeted sgRNA libraries enable high-throughput interrogation of genes involved in specific biological functions. Through functional screening, enrichment analysis, PCR amplification, and next-generation sequencing, genes associated with the phenotype of interest can be systematically identified.

• The entire service workflow strictly adheres to QC standards. The sgRNA plasmid library is maintained at a storage capacity exceeding 500× the total number of sgRNAs, and library coverage is verified by NGS sequencing to achieve over 99% representation.
• Lentiviral transduction is employed to maximize sgRNA delivery efficiency and ensure robust gene perturbation.
• Rich screening platform: control cell survival, combined with immunostaining, flow cytometry and other screening methods.
• Multiple screening approaches are available, including control of cell viability, immunostaining, flow cytometry, and other phenotypic assays, allowing flexible and precise functional screening.

CRISPR-Cas9 sgRNA Library Construction Service Process